WebCreates a Seurat object containing only a subset of the cells in the original object. Takes either a list of cells to use as a subset, or a parameter (for example, a gene), to subset on. RDocumentation. Search all packages and functions. Seurat (version 2.3.4) WebNov 29, 2024 · I have a Seurat object with 20 different groups of cells (all are defined in metadata and set as active.ident). 10 of them are "treated" and 10 are "untreated" (this info is also in metadata). R Seurat package. I am trying to make a DimPlot that highlights 1 group at a time, but the colours for "treated" and "untreated" should be different.

BimberLab/cellhashR - Github

WebUsing Seurat with multi-modal data; Analysis, visualization, and integration of spatial datasets with Seurat; Data Integration; Introduction to scRNA-seq integration; Mapping … WebThis is a convenience wrapper function around the following three functions that are often run together when mapping query data to a reference: TransferData, IntegrateEmbeddings, ProjectUMAP. Note that by default, the weight.reduction parameter for all functions will be set to the dimension reduction method used in the FindTransferAnchors function call … psbc dating tool

WhichCells function - RDocumentation

Our multiplexing strategy not only enables pooling across donors but also the simultaneous profiling of multiple experimental conditions. This is widely applicable to the simultaneous profiling of diverse environmental and genetic perturbations, but we reasoned that we could also efficiently optimize experimental … See more We sought to extend antibody-based multiplexing strategies [16, 17] to scRNA-seq using a modification of our CITE-seq method [18]. We … See more We next compared our HTO-based classifications to those obtained by demuxlet [13]. Overall, we observed a strong concordance between the techniques, even … See more For our proof of principle experiments, we used a pool of antibodies directed against highly expressed immune surface markers (CD45, CD98, CD44, and CD11a). To enable multiplexing of any cell type and sample, we decided … See more Our cell hashtags can discriminate single cells from doublets based on the clear expression of a single HTO, and we next asked whether this feature could also distinguish low … See more WebJul 25, 2024 · 这里将简要演示如何在Seurat中处理由Cell Hashing 生成的数据。. 应用于两个数据集，我们可以成功地将细胞分离到它们的原始样本，并识别出交叉样本双峰（cross-sample doublets）。. 多路复用函 … WebSeurat (version 2.3.1) Description. Usage Arguments … Value. Examples Run this code # NOT RUN {WhichCells(object = pbmc_small, ident = 2) # } Run the code above in your browser using DataCamp Workspace. Powered by ... horse riding camps for adults