Ctcf kda
WebApr 10, 2024 · It contains 37 exons that encode a 2492 amino acid protein with a molecular weight of 282.586 kDa [22,23]. Based on its ATPase/helicase C-terminal domain, the protein is a member of the SWI/SNF family. ... (CTCF, which is localized by H3.3 histones) and boundary disturbances of the chromatin loop, which promote coordinated loop-wide … WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control.
Ctcf kda
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WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. WebGeneral description. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator ...
WebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific targets of poly(ADP-ribosyl)ation in tumor development. Two forms of the multifunctional transcription factor, CTCF, were previously identified: a 130-kDa protein (CTCF-130), … WebThe recombinant protein with a predicted molecular weight of 43.3 kDa was removed from the gel and purified by electroelution. The electroelution was conducted in cellulose tubes (Sanko Junyaku Co., Ltd., Tokyo, Japan) filled with an electroelution buffer (100 mM sodium phosphate and 0.1% SDS; pH 7.0) at 100 V and 100 mA for 17 h.
WebDepartment of Children and Families. 505 Hudson Street. Hartford CT 06106. Phone Number: 860-550-6300. E-mail the Commissioner. WebCTCF has a predicted molecular weight of 84 kDa and CTCFL, 74 kDa. However, CTCF is known to migrate as a 130-kDa protein [39]. Since the transgenes are expressed as fusion proteins with FLAG tag...
WebApr 6, 2012 · Here, we report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses. We found that stimulation of human corneal …
Web~ 130 kDa Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with … carinthia カリンシア defence 6 シュラフWebJan 30, 2024 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific … cariru あんしんプランWebFeb 1, 1997 · CTCF belongs to the Zn finger transcription factors family and binds to the promoter region of c-myc. CTCF is highly conserved between species, ubiquitous and localised in nuclei. The endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a … cariot キャリオットWebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific … cariru クーポンコードWebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific … cariru レンタルWebJun 8, 2004 · The protein CTCF plays an essential role in the action of a widely distributed class of vertebrate enhancer-blocking insulators, of which the first example was found in a DNA sequence element, HS4, at the 5' end of the chicken beta-globin locus. ... a 38-kDa nucleolar phosphoprotein that is concentrated in nuclear matrix preparations. In this ... carinthia カリンシア defence 6 シュラフ マミー型 寝袋WebAbFlex® CTCF recombinant antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of MCF7 chromatin and 4 µg of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to … carista obd2 アダプタ 設定手順 取扱説明