Determination of free proline in plants
WebAccumulation of proline in higher plants is a sign of disturbed physiological condition, caused through stress condition. Free proline content can increase exposure of plants to … WebProline (Pro) accumulation is a common physiological response in many plants in response to a wide range of biotic and abiotic stresses. Controversy has surrounded the possible role(s) of proline accumulation. In this review, knowledge on the regulation of Pro metabolism during development and stres …
Determination of free proline in plants
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WebJan 1, 2024 · In plants, proline is synthesized from either glutamate or ornithine. ... (6.57 dS m −1) induced the accumulation of free proline in leaves and roots, ... Rapid determination of free proline for water – stress studies. Plant Soil, 39 (1973), pp. 205-207. Google Scholar. WebJan 1, 2011 · high throughput determination of free proline content using a microplate reader. Ninhy drin (2,2 - dihydroxyindane-1,3-dione, CAS number 485-47-2) is extensively used to assay amino acids.
WebProline is a basic amino acid found in high percentage in basic protein. Free proline is said to play a role in plants under stress conditions. Though the molecular mechanism has not yet been established for the increased level of proline, one of the hypotheses refers to breakdown of protein into amino acids and conversion to proline for storage. WebAccumulation of proline in response to environmental stresses seems tobe widespread among plants. To elucidate the role of proline in plantresponses,in vivo and in vitro, we studied theeffect of proline on ... Bates L.S., Waldren R.P. and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205–207
WebDetermination of Free Proline Content Aliquots of ground plants material is heated for 20 min in pure ethanol as well as in water. The resulting mixture was left overnight at 4°C, and centrifuged at 14000 rpm (5 min). The cold extraction procedure was repeated on the pellet and supernatants pooled and used for the analysis (Carillo et al ... WebMar 8, 2010 · Determination of free proline levels is a useful assay to monitor physiological status and to assess stress tolerance of higher plants. Here we describe three methods suitable for determination of ...
WebThe accumulation of imino acid proline in plants in response to salinity- or water stress-mediated osmotic stress has been extensively studied. ... R.P. Waldren, and L.D. Teare. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205-207. Bradford, M.M. 1976. A rapid and sensitive method for quantitative of ...
WebBates, L.W., Waldern, R.P. and Teare, I.D. (1973) Rapid Determination of Free Proline Foe Water Stress. Plant Soil, 39, 205-207. ... Chemical Constituents and Growth Yield of Maize Plant Gown under Drought Stress. AUTHORS: Hamdia Mahmoud Abd El-Samad, Khloud Nagy Shaker Abd El-Hakeem. google chrome keeps opening in frenchWebPlant material and extraction. Proline is very soluble and can be readily extracted by heating explants or aliquots of ground plant material for 20 min in pure ethanol as well as in … chicago business school executive educationWebJSTOR Home google chrome keeps opening new tabsWeb3. 2 ml of filtrate is taken in a test tube and 2 ml of glacial acetic acid and 2 ml acid-ninhydrin are added in a sequence. 4. The mixture is heated it in the boiling water-bath for 1 h. 5. The reaction is stopped by placing the tube in ice-bath. 6. 4 ml toluene is added to the reaction mixture and stir well for 20-30 sec. chicago bus rental companiesWebProline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for … chicago bus map routeWebJan 1, 2016 · With a greater accumulation in salt tolerant plants, proline concentrations markedly increased in both salt sensitive and tolerant plants grown under salinity stress (Mansour et al., 2005). ... Rapid determination of free proline for water-stress studies. Plant and Soil, 39 (1973), pp. 205-207. Google Scholar. google chrome keeps randomly quittingWebAccumulation of proline in higher plants is an indication of disturbed physiological condition, triggered by biotic or abiotic stress condition. Free proline content can increase upon exposure of plants to drought, … google chrome keeps opening by itself